Nb. Pestov et al., Immunochemical demonstration of a novel beta-subunit isoform of X,K-ATPasein human skeletal muscle, BIOC BIOP R, 277(2), 2000, pp. 430-435
Citations number
24
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Recently we have identified mRNA encoding a hitherto unknown mammalian X,K-
ATPase beta -subunit expressed predominantly in muscle tissue (Pestov, N. B
. ef al. (1999) FEES Lett. 456, 243-248), Here we demonstrate the existence
of the predicted protein, designated as beta (m) (beta (muscle)), in human
adult skeletal muscle membranes using immunoblotting with beta (m)-specifi
c antibodies generated against recombinant polypeptide formed by extramembr
ane beta (m) domains. The electrophoretic mobility of beta (m) was shown to
be abnormally low due to the presence of Glu-rich sequences, In contrast t
o mature forms of other known X,K-ATPase beta -subunits, carbohydrate moiet
y of beta (m) is sensitive to endoglycosidase H and appears to be composed
of short high-mannose or hybrid N-glycans, This finding argues in favor of
an intracellular location of beta (m) in human skeletal muscle. (C) 2000 Ac
ademic Press.