M. Yanase et al., Lysophosphatidic acid enhances collagen gel contraction by hepatic stellate cells: Association with Rho-kinase, BIOC BIOP R, 277(1), 2000, pp. 72-78
Citations number
45
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
We studied the effect of lysophosphatidic acid (LPA) on collagen gel contra
ction by cultured rat hepatic stellate cells (HSCs) in association with the
function of Rho-kinase, one of the target molecules of small GTPase Rho, B
inding studies showed a single class-binding site of LPA on HSCs. LPA enhan
ced the contraction of a collagen lattice seeded with HSCs, LPA increased t
he number of HSCs with polygonal morphology that contained actin stress fib
ers, and enhanced the phosphorylation of myosin light chain and the assembl
y of focal adhesion kinase and RhoA around fibronectin-coated beads seeded
on HSCs. The electric cell-substrate impedance sensor system showed that LP
A enhanced adhesion of HSC to extracellular substrate. All the effects of L
PA were suppressed by Y-27632, Rho-kinase inhibitor. These data support the
notion that LPA is involved in modulating HSC morphology, its attachment t
o surrounding extracellular matrix and its contraction by a mechanism invol
ving Rho-kinase. (C) 2000 Academic Press.