Tp. Dooley et al., Expression profiling of human sulfotransferase and sulfatase gene superfamilies in epithelial tissues and cultured cells, BIOC BIOP R, 277(1), 2000, pp. 236-245
Citations number
57
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
The bioavailability of drugs administered topically or orally depends on th
eir metabolism by epithelial enzymes such as the cytosolic sulfotransferase
s (SULT). Reverse transcriptase-polymerase chain reaction (RT-PCR) methods
were established to detect expression of 8 SULT genes and 4 arylsulfatase (
ARS) genes in human tissues of epithelial origin and in cultures of normal
and transformed (cancer) cells. The results indicate: (i) SULT 1A1, 1A3, AR
SC, and ARSD genes are ubiquitously expressed; (ii) expression is frequentl
y similar between cell lines and corresponding tissues; (iii) SULT gene exp
ression in normal cultured cells is generally comparable to the expression
in associated transformed (cancer) cell lines; (iv) SULT 1A1 promoter usage
is mainly tissue specific; however, both promoters are frequently used in
SULT 1A3 expression; and (v) the expression profile of SULT 1A1, 1A3, 1E1,
and 2B1a/b suggests that one or more of these isoforms may be involved in t
he cutaneous sulfoconjugation of minoxidil and cholesterol. (C) 2000 Academ
ic Press.