Zl. Chen et al., Separation of hydroxy acid enantiomers by ligand exchange-micellar electrokinetic chromatography, BUNSEKI KAG, 49(10), 2000, pp. 759-764
Hydroxy acid enantiomers have specific bioactivity. Much attention has been
paid to their analytical separation. This work investigated their separati
on and behavior by ligand exchange-micellar electrokinetic chromatography u
sing Cu(II)-L-OH-proline complexes as chiral selectors. Previous work showe
d that D-amino acid enantiomers migrated faster than L-analogues when Cu(II
)-L-proline complexes were used as chiral selectors. The enantiomer migrati
on orders (EMO) of amino acids were reversed by introducing the SDS micella
r phase. In the case of hydroxy acid enantiomers, however, it was observed
that L-enantiomers migrated faster than the D-forms in both cases of withou
t and with the SDS micellar phase. When CTAB was added to running electroly
tes, D-enantiomers migrated as the first peak. Further, when the ligands we
re changed, different EMOs were observed. The resolution condition, mechani
sm and behaviors are discussed in this paper.