Centromeric heterochromatin in the cattle rob(1;29) translocation: alpha-satellite I sequences, in-situ MspI digestion patterns, chromomycin stainingand C-bands

The centromeric regions and alpha -satellite I sequence were studied on chr omosomes 1, 29 and the rob(1;29) translocation in a Portuguese breed of cat tle, Barrosa, carrying the translocation. Rob(1;29) centromeric regions sho wed heterochromatic bands with propidium iodide but, unlike the acrocentric autosomes, no strong centromeric bands were revealed with chromomycin A3. An alpha -satellite I sequence was not found at the centromeres of the X, Y and rob(1;29) chromosomes in the breed, although it was present at the cen tromeres of all acrocentric chromosomes including 1 and 29. Restriction enz yme banding with MspI revealed polymorphisms between different rob(1;29) ch romosomes in both centromeric and intercalary regions. The data show that t he centromeric region of the rob(1;29) chromosome has lost the alpha -satel lite I sequences, while retaining other heterochromatin, and suggest that t his important and widespread translocation has occurred multiple times.