Functional analysis of the leader peptide of the yeast gene CPA1 and heterologous regulation by other fungal peptides

Citation
P. Delbecq et al., Functional analysis of the leader peptide of the yeast gene CPA1 and heterologous regulation by other fungal peptides, CURR GENET, 38(3), 2000, pp. 105-112
Citations number
22
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CURRENT GENETICS
ISSN journal
01728083 → ACNP
Volume
38
Issue
3
Year of publication
2000
Pages
105 - 112
Database
ISI
SICI code
0172-8083(200010)38:3<105:FAOTLP>2.0.ZU;2-D
Abstract
The 25-amino-acid leader peptide present at the 5' end of yeast CPA1 mRNA i s responsible for the translational repression of that gene by arginine. We show here that the active domain of the yeast peptide is highly specific a nd extends over amino acids 6-23. The region between amino acids 6-21 is we ll conserved between similar peptides present upstream of CPA1-homologous g enes in other fungi. The Neurospora crassa arg-2 peptide represses the expr ession of CPA1, whereas the peptide from Aspergillus nidulans has only a we ak regulatory effect. Such results suggest that the N- and C-terminal amino acids of the peptide may influence its regulatory activity. We also show t hat the transcription start sites of CPA1 are not modified when the cells a re grown in the presence of arginine, nor in a strain carrying an inactive peptide.