The hypothesis that growth hormone (GH) can affect immune responses in man
has been evaluated by monitoring cytokine expression in cultures from perip
heral blood mononuclear cells, by enzyme-linked immunosorbent assay (ELISA)
and ribonuclease protection assay, and in tonsillar cells by ELISA. In add
ition to pituitary GH (GH-N), the placental form (GH-V), differing from pit
uitary GH by 13 amino acids has also been tested. Only few effects reached
statistical significance and were in no case greater than 15%. Pituitary GH
slightly reduced IL-5 production and stimulated IFN-gamma production. The
latter effect was also observed with prolactin and could thus be induced th
rough the prolactin receptor. It is proposed that GH has no strong effects
on the parameters investigated, possibly as a result of redundancy in the c
ytokine network. Alternatively, effects on leukocytes are mediated by other
tissues such as the liver or are clear only in response to stronger challe
nges.