PARTIAL-PURIFICATION OF HLAMP-1 PROVIDES DIRECT EVIDENCE FOR THE MULTICOMPONENT NATURE OF THE PARTICULATE MATRIX ASSOCIATED WITH CARDIAC MESENCHYME FORMATION

H-LAMP-1 is a 283 kDa protein that is involved in the transformation o f endothelial cells into mesenchyme within the AV canal and proximal o utflow tract of the heart. This protein is part of the particulate mat rix that has been suggested to be composed of multicomponent complexes that have been termed cardiac adherons. However, to date no direct ev idence has been provided that these proteins are complexed into an adh eron-like particle. This report provides the first such evidence by sh owing that purification of hLAMP-1, under gentle conditions, results i n the isolation of multiple bands of similar molecular weight within t he fractions that contain anti-hLAMP-1 activity. (C) 1997 Wiley-Liss, Inc.