M. Triantafilou et al., A 70 kDa MHC class I associated protein (MAP-70) identified as a receptor molecule for Coxsackievirus A9 cell attachment, HUMAN IMMUN, 61(9), 2000, pp. 867-878
One of the major categories of disease-causing micro-organisms are viruses.
New studies on many different viruses have shown that virus attachment and
cell entry is often a multistep process, requiring many interactions betwe
en the virus and cell surface molecules. In this study, we have attempted t
o identify the cell surface molecules involved in Coxsackievirus A9 (CAV-9)
, a common human pathogen and a member of the Picornavirus family, infectio
us process. GMK cells susceptible to virus infection were surfaced labeled
with biotin and then solubilized in non-ionic and zwiterionic detergents. F
ree CAV-9 virions were used as an affinity surface, allowing the virus to b
ind to the solubilized receptors. The virus-receptor complexes were then im
munoprecipitated by an anti CAV-9 serum and protein-A sepharose beads. SDS-
PAGE and two-dimensional electrophoresis revealed the presence of integrin
alphav beta3 molecules and a 70 kDa protein with apparent isoelectric point
(pI) 5.5. The identity of the integrin alphav beta3 molecules was confirme
d by immunoprecipitation and Western blotting; whereas the 70 kDa protein w
as also found ro co-immunoprecipitate with MHC class I molecules in non-str
ingent conditions. Sequential immunoprecipitation experiments confirmed tha
t the MHC class I associated protein (MAP-70) and the 70 kDa protein utiliz
ed by CAV-9 were identical. The role of MAP-70 in CAV-9 infectious process
is discussed. Human Immunology 61, 867-878 (2000). (C) American Society for
Histocompatibility and Immunogenetics, 2000. Published by Elsevier Science
Inc.