H. Nakano et al., ACCUMULATION OF TRANSLATIONAL INHIBITOR DURING MULTI-HOUR CELL-FREE PROTEIN-SYNTHESIS REACTION USING RABBIT RETICULOCYTE LYSATE, Journal of fermentation and bioengineering, 83(5), 1997, pp. 470-473
A cell-free protein synthesis in rabbit reticulocyte lysates stops wit
hin 2 h, which limits the productivity of the cell-free system. Here w
e show that the accumulation of a translational inhibitor liberated fr
om the post-ribosomal supernatant during the reaction time is responsi
ble for the halt in the translational reaction. Since EGTA was found t
o suppress the effect of the inhibitor and the absorbance of a Ca2+ in
dicator was observed to increase, Ca2+ is suggested to be the inhibito
r that accumulates in reticulocyte lysates during the cell-free protei
n synthesis reaction.