T. Tanase et al., COMPARISON OF MICRO-FILTRATION HOLLOW-FIBER BIOREACTORS FOR MAMMALIAN-CELL CULTURE, Journal of fermentation and bioengineering, 83(5), 1997, pp. 499-501
The production of tissue plasminogen activator (tPA) in various micro-
filtration hollow fiber (MFHF) bioreactors for mammalian cell culture
was compared. When the surface area of the bioreactor was increased 3-
fold, tPA production per m(2) decreased 30%. Changing the angle of the
medium distributor at the inlet of the hollow fiber cartridge increas
ed the tPA production per m(2) by 15%. tPA production reached a maximu
m at 20 d, but then decreased gradually. In order to maintain the tPA
production over a long period, a cartridge whose surface area was redu
ced by 20% by decreasing the number of hollow fibers was utilized. tPA
was produced stably, and the tPA production per m(2) was 238 mg/d/m(2
). Cumulative tPA production for 50 d reached 19 g, and was almost equ
al to that with the previous cartridge. It was concluded that the MFHF
bioreactor can be scaled up if a uniform and sufficient supply of oxy
gen is achieved and good physiological conditions for mammalian cells
are maintained.