High-performance liquid chromatographic determination of vinorelbine in human plasma and blood: application to a pharmacokinetic study

A sensitive and specific high-performance liquid chromatographic method wit h fluorescence detection (excitation wavelength: 280 nm; emission wavelengt h: 360 nm) was developed and validated for the determination of vinorelbine in plasma and blood samples. The sample pretreatment procedure involved tw o liquid-liquid extraction steps. Vinblastine served as the internal standa rd. The system uses a Spherisorb cyano analytical column (250X4.6 mm I.D.) packed with 5 mum diameter particles as the stationary phase and a mobile p hase of acetonitrile-80 mM ammonium acetate (50:50, v/v) adjusted to pH 2.5 with hydrochloric acid. The assay showed linearity from 1 to 100 ng/ml in plasma and from 2.5 to 100 ng/ml in blood. The limits of quantitation were 1 ng/ml and 2.5 ng/ml, respectively. Precision expressed as RSD was in the range 3.9 to 20% (limit of quantitation). Accuracy ranged from 92 to 120%. Extraction recoveries from plasma and blood averaged 101 and 75%, respectiv ely. This method was used to follow the time course of the concentration of vinorelbine in human plasma and blood samples after a 10-min infusion peri od of 20 mg/m(2) of this drug in patients with metastatic cancer. (C) 2000 Elsevier Science B.V. All rights reserved.