Construction of a full-length infectious cDNA clone of swine vesicular disease virus strain NET/1/92 and analysis of new antigenic variants derived from it

Citation
Jmj. Rebel et al., Construction of a full-length infectious cDNA clone of swine vesicular disease virus strain NET/1/92 and analysis of new antigenic variants derived from it, J GEN VIROL, 81, 2000, pp. 2763-2769
Citations number
25
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF GENERAL VIROLOGY
ISSN journal
00221317 → ACNP
Volume
81
Year of publication
2000
Part
11
Pages
2763 - 2769
Database
ISI
SICI code
0022-1317(200011)81:<2763:COAFIC>2.0.ZU;2-3
Abstract
The Dutch swine vesicular disease virus (SVDV) isolate NET/1/92 was one of the first isolates belonging to a new SVDV antigenic group. This strain was completely sequenced and was shown to have 93% similarity with the UKG/27/ 72 isolate. To enable antigenicity, replication, maturation and pathogenici ty studies of NET/1/92, an infectious full-length cDNA clone, designated pS VD 146, was prepared. The in vitro and in vivo biological properties of the virus derived from pSVD 146 were studied by analysing antigenicity, plaque morphology, growth curves and virulence in pigs. The epitopes of newly pre pared monoclonal antibodies were roughly mapped by fusion-PCR. Fine mapping of epitopes at the amino acid level was achieved by introducing single ami no acid mutations in pSVD 146. Two new amino acids important in epitope for mation were located in VP1; one was mapped in the C-terminal end and the se cond is thought to be located in the H-1 loop. Growth curve and plaque size s in vitro were similar between virus derived from pSVD146 and the parent w ild-type virus. In virulence studies in pigs, the lesions score, neutraliza tion titres and the seroconversion rates were comparable between virus deri ved from pSVD146 and the parent strain. Since virus derived from pSVD146 ha d the same biological properties as the parent strain NET/1/92, the full-le ngth infectious cDNA clone pSVD146 will be very useful in studies of the an tigenicity, virulence, pathogenesis, maturation and replication of SVDV.