Me. Miranda-carus et al., Anti-SSA/Ro and anti-SSB/La autoantibodies bind the surface of apoptotic fetal cardiocytes and promote secretion of TNF-alpha by macrophages, J IMMUNOL, 165(9), 2000, pp. 5345-5351
Despite the near universal association of congenital heart block and matern
al Abs to SSA/Ro and SSB/La, the intracellular location of these Ags has ma
de it difficult to substantiate their involvement in pathogenicity. To defi
ne whether components of the SSA/Ro-SSB/La complex, which translocate durin
g apoptosis, are indeed accessible to extracellular Abs, two approaches wer
e taken: immunoprecipitation of surface biotinylated proteins and scanning
electron microscopy. Human fetal cardiocytes from 16-24-wk abortuses were c
ultured and incubated with staurosporine to induce apoptosis, Surface bioti
nylated 48-kDa SSB/La was reproducibly immunoprecipitated from apoptotic, b
ut not nonapoptotic cardiocytes, Surface expression of SSA/Ro and SSB/La wa
s further substantiated by scanning electron microscopy, Gold particles (fo
llowing incubation with gold-labeled sera containing various specificities
of anti-SSA/Ro-SSB/La Abs and murine mAb to SSB/La and 60-kDa SSA/Ro) were
consistently observed on early and late apoptotic cardiocytes. No particles
were seen after incubation with control antisera. To evaluate whether opso
nized apoptotic cardiocytes promote inflammation, cells were cocultured wit
h macrophages. Compared with nonapoptotic cardiocytes or apoptotic cardiocy
tes incubated with normal sera, apoptotic cardiocytes preincubated with aff
inity-purified Abs to SSB/La, 52-kDa SSA/Ro, or 60-kDa SSA/Ro increased the
secretion of TNF-alpha from cocultured macrophages. In summary, apoptosis
results in surface accessibility of all SSA/Ro-SSB/La Ags for recognition b
y circulating maternal Abs. It is speculated that in vivo such opsonized ap
optotic cardiocytes promote an inflammatory response by resident macrophage
s with damage to surrounding conducting tissue.