Antigenic conservation of an immunodominant invariable region of the VlsE lipoprotein among European pathogenic genospecies of Borrelia burgdorferi SL

Lyme disease is caused by genetically divergent spirochetes, including 3 pa thogenic genospecies: Borrelia burgdorferi sensu stricto, B. garinii, and B . afzelii. Serodiagnosis is complicated by this genetic diversity. A synthe tic peptide (C-6), based on the 26-mer invariable region (IR6) of the varia ble surface antigen of B. burgdorferi (VlsE), was used as ELISA antigen, to test serum samples collected from mice experimentally infected with the 3 genospecies and from European patients with Lyme disease, Regardless of the infecting strains, mice produced a strong antibody response to C-6, which indicates that IR6 is antigenically conserved among the pathogenic genospec ies. Twenty of 23 patients with culture-confirmed erythema migrans had a de tectable antibody response to C-6, A sensitivity of 95.2% was achieved, wit h serum samples collected from patients with well-defined acrodermatitis ch ronica atrophicans, Fourteen of 20 patients with symptoms of late Lyme dise ase also had a positive anti-IR6 ELISA, Thus, it is possible that C-6 may b e used to serodiagnose Lyme disease universally.