Angiotensin II induces nuclear factor-kappa B activation in cultured neonatal rat cardiomyocytes through protein kinase C signaling pathway

Rat neonatal ventricular cardiomyocytes (RNVM) possess G protein-coupled AT (1) receptors for angiotensin II (AngII) that activate multiple intracellul ar pathways. To elucidate potential signaling mechanisms involved, we focus sed on the nuclear transcription factor-kappa B (NF-kappaB) in RNVM culture . Using specific antibody to NF-kappa Bp65, immunolocalization of NF-kappaB was cytoplasmic in unstimulated cardiomyocytes, whereas NF-kappaB was tran slocated into the RNVM nucleus in response to AngII. This translocation was inhibited in the presence of calphostin C, a specific inhibitor of protein kinase C (PKC). Western blot analysis showed an increase of NF-kappaB in A ngII-stimulated cardiomyocyte nuclear extracts as compared to controls, Bio molecular interaction analysis (BIA analysis) of NF-kappaB activation showe d that only AngII-nuclear extracts bound to NF-kappaB consensus sequence wi th a high degree of affinity. This DNA-binding capacity was completely lost in calphostin C-treated cells. At transcriptional level in RNVM, AngII med iates the upregulation of matrix gelatinase (MMP-9), which is totally inhib ited by calphostin C treatment. In conclusion, cardiomyocyte nuclear NF-kap paB translocation in response to Ang II via PKC pathway activates cardiomyo cyte-specific transcription of MMP-9 and may activate transcription From re sponsive genes which are involved in cardiac hypertrophy process and/or car diac remodeling. (C) 2000 Academic Press.