The in vitro ethanol sensitivity of hippocampal synaptic gamma-aminobutyric acid(A) responses differs in lines of mice and rats genetically selected for behavioral sensitivity or insensitivity to ethanol

Previous work has demonstrated that in the hippocampal CA1 region of Spragu e-Dawley rats, there are ethanol-sensitive and ethanol-insensitive populati ons of GABAergic synapses on pyramidal neurons. The present experiments cha racterized the ethanol sensitivity of these pathways in lines of rats and m ice genetically selected for sensitivity or insensitivity to the behavioral effects of ethanol. In ethanol-sensitive inbred long sleep mice, GABA(A) I PSCs induced by stimulation of proximal (probably somatic) synapses were en hanced by 80 mM ethanol, whereas the distal (i.e., dendritic) pathway was u naffected. Thus, the relative sensitivity of these pathways (proximal. dist al) is the same in both Sprague-Dawley rats and in inbred long sleep mice. However, in the ethanol-insensitive inbred short sleep mice, neither proxim al nor distal IPSCs were affected by 80 mM ethanol. The ethanol sensitivity of the proximal pathway was also examined in replicate lines of rats selec ted for either high ethanol sensitivity or low ethanol sensitivity. GABA(A) IPSCs in the high ethanol sensitivity lines were significantly enhanced by 80 mM ethanol, whereas IPSCs in the low ethanol sensitivity lines were una ffected. Thus, IPSCs evoked via the proximal pathway were enhanced by ethan ol in all the sensitive mouse and rat lines, and unaffected in all the inse nsitive lines. These experiments demonstrate that GABA(A) synapses in brain differ in their sensitivity to enhancement by ethanol, and the sensitivity to such enhancement is under the control of genes that can be selected for using classical genetic selective breeding based on a behavioral phenotype .