Integration of macromolecular diffraction data using radial basis functionnetworks

This paper presents a novel approach for intensity calculation of X-ray dif fraction spots based on a two-stage radial basis function (RBF) network. Th e first stage uses pre-determined reference profiles from a database as bas is functions in order to locate the diffraction spots and identify any over lapping regions. The second-stage RBF network employs narrow basis function s capable of local modifications of the reference profiles leading to a mor e accurate observed diffraction spot approximation and therefore accurate d etermination of spot positions and integrated intensities.