Amphiphilic polymer-liposome interaction: a novel immobilization techniquefor liposome on gel surface

We developed a simple method for the immobilization of liposomes and biolog ical membrane fragments to gel surface using an amphiphilic polymer, consis ting of octadecylacrylamide and N,N-dimethylacrylamide with a molar ratio O f 99:1. Calcein-entrapped egg phosphatidylcholine liposomes were successful ly immobilized to the surface of polyacrylamide gel containing the polymer without significant loss of entrapped calcein, Different contents of the am phiphilic polymer in the gel resulted in the different surface density of i mmobilized liposomes on the surface of the gel in a dose-dependent manner. As an example of biological membranes, microsome membranes from yeast cells were also well immobilized to the gel. (C) 2000 Elsevier Science S.A. All rights reserved.