Assessment of a mono clonal antibody-based competitive enzyme linked immunosorbent assay (cELISA) for diagnosis of brucellosis in infected and Rev. 1vaccinated sheep and goats

In this study a cELISA for the diagnosis of brucellosis due to B. melitensi s in sheep and goats was evaluated and its capability of discriminating vac cinated from infected animals was assessed. Information is provided indicat ing that the cELISA has a diagnostic sensitivity (99.4%) and specificity (9 8.9%) in sheep and goats comparable to that of many standard indirect ELISA methods. In addition, the test proved able to distinguish between vaccinat ed and infected animals with an accuracy of up to 90% and results reproduci bility of 93%. It was concluded that the cELISA could be useful for differe ntiation of Rev.1 vaccinated and naturally infected sheep and goats.