Iv. Korobko et al., Stromal cells paracrinously regulate the c-met transcript in VMR tumor cells of different in metastatic potential, MOL BIOL, 34(5), 2000, pp. 648-651
The c-met protooncogene, which codes for tyrosine protein kinase, was shown
to be more intensely transcribed in highly metastatic tumor VMR-P than in
low-grade VMR-0. The gene for the hepatocyte growth factor (HGF), a c-Met l
igand, was transcribed in VMR-P but not in VMR-0 tumor. The content of the
c-met mRNA was the same and HGF was not transcribed in VMR-0 and VMR-P cell
s cultured in vitro. Thus, the difference between VMR-P and VMR-0 tumors wa
s attributed to interactions of tumor and stromal cells. The in vitro cocul
turing of tumor cells and fibroblasts confirmed paracrine regulation of c-m
et transcription.