Millimolar concentrations of methylmethanesulfonate enhance transcription from the cytomegalovirus early promoter at least 100-fold

Authors
Nabirochkina, EN Ivanov, AV Soldatov, AV
Citation
En. Nabirochkina et al., Millimolar concentrations of methylmethanesulfonate enhance transcription from the cytomegalovirus early promoter at least 100-fold, MOL BIOL, 34(5), 2000, pp. 652-654
Citations number
11
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
34
Issue
5
Year of publication
2000
Pages
652 - 654
Database
ISI
SICI code
0026-8933(200009/10)34:5<652:MCOMET>2.0.ZU;2-#
Abstract
Treatment of cultured cells with methylmethanesulfonate enhanced transcript ion from the CMV early promoter 25-250 times and protein synthesis 60-100 t imes in a dose-dependent way. The effect was promoter-specific and was not observed with a retroviral LTR promoter. Structural analogs such as ethylme thanesulfonate, trifluoromethanesulfonate, and p-toluenesulfonate did not e nhance expression from the CMV promoter.