Es. Ioudinkova et Sv. Razin, Study of the regulatory sequences associated with DNase I-hypersensitive sites in the 5 ' region of the chicken alpha-globin gene domain, MOL BIOL, 34(5), 2000, pp. 696-701
The functional activity of DNA sequences from the 5' region of the chicken
alpha -globin gene domain was studied. Silencers and transcription terminat
ors were found in a fragment located 11-14.5 kb upstream of the pi gene. A
fragment located 3-4.5 kb upstream of pi was shown to contain a CpG cluster
, a part of which proved to be selectively methylated in nonerythroid cells
. Cloned upstream of the alphaD gene promoter, this frap ment suppressed th
e promoter activity when all its CpG were methylated.