Cloning and characterization of RAPD markers of parasitic nematodes Trichinella spiralis and T-pseudospiralis

Authors
Chrisanfova, GG Semyenova, SK Ryskov, AP
Citation
Gg. Chrisanfova et al., Cloning and characterization of RAPD markers of parasitic nematodes Trichinella spiralis and T-pseudospiralis, MOL BIOL, 34(5), 2000, pp. 702-706
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
34
Issue
5
Year of publication
2000
Pages
702 - 706
Database
ISI
SICI code
0026-8933(200009/10)34:5<702:CACORM>2.0.ZU;2-6
Abstract
A RAPD analysis was carried out in parasitic nematodes Trichinella spiralis and T. pseudospiralis, which are among the most common and hazardous agent s in human and animal helminthoses. Several RAPD fragments were cloned and some of them sequenced. The fragments were shown to correspond to unique or low-repetitive sequences and to contain ORFs, microsatellites, and regions homologous to various pro- and eukaryotic sequences. Several fragments can be used to distinguish between T. spiralis and T. pseudospiralis.