Ng. Kan et al., Detection of genetically unstable loci in parthenogenic families of lizards of the Lacerta genus by DNA fingerprinting, MOL BIOL, 34(5), 2000, pp. 707-711
Two parthenogenic families of unisexual species of Caucasian rock lizards o
f genus Lacerta, L. armeniaca and L. unisexualis, were analyzed by DNA fing
erprinting. Inheritance of M13 minisatellite and of (GACA)(n), (GATA)(n), a
nd (TCC)(n), microsatellite loci in the first generation of the lizards was
studied. M13, (GBCA)(n), and (TCC)(n) loci in the families oft. armeniaca
were strictly inherited, as well as M13 and (GACA)(n) loci in the families
of L. unisexualis: each DNA fragment in the fingerprint patterns of progeny
could be detected in the maternal pattern. However, when a (TCC)SO microsa
tellite probe was applied in the study of L. unisexualis families, specific
DNA fragments with altered mobility were revealed in the progeny patterns,
and the frequency of such events was rather high. It might be hypothesized
that some of the (TCC)(n) loci in L. unisexualis genome are highly mutable
. Hence, the family analysis allowed us to demonstrate experimentally the p
resence of genetically unstable loci in genomes of parthenogenic species of
vertebrates. The nature and mechanism of the instability of these loci in
parthenogenesis remain obscure.