Am. Gutierrez et al., The effect of lindane on the lipid peroxidation of microsomes and mitochondria isolated from liver and heart of Columba livia, PEST BIOCH, 68(2), 2000, pp. 119-126
The effect of lindane treatment on the nonenzymatic lipid peroxidation of m
icrosomes and mitochondria isolated from liver and heart of the rock dove,
Columba livia, was studied. Fatty acid composition of liver was drastically
changed by lindane treatment. Percentages of C18:1n9 and C16:1n7 fairy aci
ds strongly decreased and percentages of 20:4n6 significantly increased. An
increase of C18:2n6 and C22:6n3 was also observed in pigeon liver microsom
es after lindane treatment After incubation of liver microsomes or mitochon
dria in an ascorbate-Fe2+ system it was observed that the total cpm/mg prot
ein originated from light emission: chemiluminescence was significantly hig
her in the lindane group than in the control. Lindane at a dose of 50 mg/kg
body weight for a period of 6 days increased the liver microsomal content
of cytochrome p450 by 100%. As compared with native, in peroxidized organel
les the values of C18:2n6 and C20:4n6 decreased significantly. These change
s were more pronounced in organelles derived from lindane-treated doves. Ch
anges in light emission and in fatty acid composition were nor observed whe
n heart mitochondria obtained from control and lindane groups were lipid pe
roxidized. However, fatty acid composition of heart microsomes was drastica
lly changed by lindane treatment. Thus, percentages of C18:1n9 and C16:1n7
fatty acids strongly decreased and percentages of 18:2n6 significantly incr
eased. After incubation of heart microsomes in an ascorbate-Fe2+ system it
was observed that the total cpm/mg protein originated from light emission:
chemiluminescence was significantly higher in the lindane group than in the
control, with a considerable decrease of linoleic acid C18:2n6, (C) 2000 A
cademic Press.