CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF THE N-TERMINAL ACTIN-BINDING DOMAIN OF HUMAN FIMBRIN

We have crystallized the N-terminal actin binding domain (ABD1) of hum an fimbrin, a representative member of the largest class of actin cros slinking proteins, Diffraction from these crystals is consistent with the orthorhombic space group P2(1)2(1)2(1) (a = 50.03 Angstrom, b = 61 .24 Angstrom, c = 102.30 Angstrom). These crystals contain one molecul e in the asymmetric unit and diffract to at least 1.9 Angstrom resolut ion. The crystal structure of ABD1 will be the first structure of an a ctin crosslinking domain. (C) 1997 Wiley-Liss, Inc.