CRYSTALLIZATION AND PRELIMINARY CRYOGENIC X-RAY-DIFFRACTION ANALYSES OF PROTEIN L-ISOASPARTYL O-METHYLTRANSFERASE FROM HUMAN PETAL BRAIN

Authors
SMITH CD BARCHUE J MENTEL C DELUCAS L SHIRASAWA T CHATTOPADHYAY D
Citation
Cd. Smith et al., CRYSTALLIZATION AND PRELIMINARY CRYOGENIC X-RAY-DIFFRACTION ANALYSES OF PROTEIN L-ISOASPARTYL O-METHYLTRANSFERASE FROM HUMAN PETAL BRAIN, Proteins, 28(3), 1997, pp. 457-460
Citations number
25
Categorie Soggetti
Biology
Journal title
Proteins → ACNP
ISSN journal
08873585
Volume
28
Issue
3
Year of publication
1997
Pages
457 - 460
Database
ISI
SICI code
0887-3585(1997)28:3<457:CAPCXA>2.0.ZU;2-Z
Abstract
Recombinant human fetal brain protein L-isoaspartyl O-methyltransferas e, EC 2.1.1.77, was crystallized in PEG 8000 with adenosine homocystei ne by a macroseeding technique, The space group was P2(1) with a = 47. 4 Angstrom, b = 53.9 Angstrom, c = 48.7 Angstrom and beta = 116.4 degr ees for cryofrozen crystals at 90 K. The crystals diffracted to 2.1 An gstrom and have one molecule per asymmetric unit. (C) 1997 Wiley-Liss, Inc.