Effect of temperature on DNA fractionation in slalom chromatography

Slalom chromatography is an alternative chromatographic procedure for the a nalysis of relatively large double-stranded DNA molecules and is based on a hydrodynamic principle. The retardation of the DNA fragments from the clea vage of the Lambda DNA by the KpnI restriction enzyme was studied using an acetonitrile-phosphate buffer as a mobile phase (flow rate equal to 0.3 ml/ min) and a C1 column as a stationary phase at various temperatures. It was shown that the temperature constituted an important parameter for the separ ation of the DNA fragments in slalom chromatography. The DNA hydrodynamic b ehavior with the temperature was related to the variation in the fluid visc osity and the modification of the elastic properties of the biopolymer. (C) 2000 Elsevier Science B.V. All rights reserved.