T. Kawahara et S. Taketa, Fixation of translocation 2A center dot 4B infers the monophyletic origin of Ethiopian tetraploid wheat, THEOR A GEN, 101(5-6), 2000, pp. 705-710
Analysis of structural chromosomal polymorphism revealed the presence of a
previously reported 2A.4B translocation common to all 15 strains of Ethiopi
an tetraploid wheat examined. Using the C-banding technique, we found two n
ew translocations, T1B.6B and T5B.6B, and a pericentric inversion of chromo
some 5A. The C-banding pattern indicated that in all three translocations t
he breakpoint was located in the centromeric region. Sequential N-banding a
nd genomic in situ hybridization (GISH) confirmed the location of the break
point of translocation 2A.4B, and revealed that the breakpoint of another k
nown translocation, 2A-2B, was in the proximal region of 2BL. The fixation
of the 2A.4B translocation indicates the monophyletic origin of Ethiopian t
etraploid wheat and the presence of a very severe bottleneck effect during
its dispersal.