Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon

Citation
J. Garcia-mas et al., Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon, THEOR A GEN, 101(5-6), 2000, pp. 860-864
Citations number
23
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
THEORETICAL AND APPLIED GENETICS
ISSN journal
00405752 → ACNP
Volume
101
Issue
5-6
Year of publication
2000
Pages
860 - 864
Database
ISI
SICI code
0040-5752(200010)101:5-6<860:CARARM>2.0.ZU;2-3
Abstract
Three different types of molecular markers, RAPD, AFLP and RFLP were used t o measure genetic diversity among six genotypes of Cucumis melo L. Each lin e represented a different melon genotype: Piel de Sapo, Ogen, PI161375, PI4 14723, Agrestis and C105. A number of polymorphic RAPD, AFLP and RFLP bands were scored on all materials and the genetic similarity measured. Clusteri ng analysis performed with the three types of markers separated the genotyp es into two main groups: (I) the sweet type, cultivated melons and (2) the exotic type, not cultivated melons. While the data obtained suggest that al l three types of markers are equally informative, AFLPs showed the highest efficiency in detecting polymorphism.