Mapping a high oleic acid mutation in winter oilseed rape (Brassica napus L.)

Two winter oilseed rape mutant lines, 7488 and 19661, with a high oleic (HO ) acid content in the seed oil were characterized phenotypically. In both m utant lines the HO trait was monogenically inherited. Segregation analysis in an F-2 population derived from a cross between 7488 and 19661 showed the two mutations to be allelic. From a comparison of seed, leaf and root fatt y acid composition it was concluded that fad2, the endoplasmic oleic acid d esaturase, is affected by the mutation. In a bulked segregant analysis thre e AFLP markers linked to this mutation were detected and localized on the g enetic map of Brassica napus. The markers mapped near the locus of one copy of the fad2 gene in the rapeseed genome.