The first linkage map established by Lanaud et al. (1995) was used as a sta
rting point to produce a high-density molecular linkage map. A mapping popu
lation of 181 progenies resulting from a cross between two heterozygous gen
otypes, a Forastero and a Trinitario (hybrid between Forastero and Criollo)
, was used for the linkage analysis. A new DNA isolation protocol was estab
lished, which allows enough good quality DNA to construct a genetic map wit
h PCR-based markers. The map comprises 424 markers with an average spacing
between markers of 2.1 cM. The marker types used were five isozymes, six lo
ci from known function genes, 65 genomic RFLPs, 104 cDNA RFLPs, three telom
eric probes, 30 RAPDs, 191 AFLPs and 20 microsatellites. The use of new mar
ker types, AFLP and microsatellites, did not disturb the original order of
the RFLP loci used on the previous map. The genetic markers were distribute
d over ten linkage groups and cover 885.4 cM. The maximum distance observed
between adjacent markers was 16.2 cM, and 9.4% of all loci showed skewed s
egregation.