K. Ohtsu et al., CLINICAL INVESTIGATION OF NEUROBLASTOMA WITH PARTIAL DELETION IN THE SHORT ARM OF CHROMOSOME-1, Clinical cancer research, 3(7), 1997, pp. 1221-1228
Several loci on the short arm of chromosome 1 (1p) have been reported
as the consensus deleted regions for the putative suppressor genes of
neuroblastoma by deletion mapping. The significance of deletion in 1p
on the clinical features of neuroblastoma remains controversial. To cl
arify the relationship between the clinical features of neuroblastoma
cases and genetic status of 1p, we performed deletion mapping on 1p on
samples obtained from 58 cases with neuroblastoma using 12 highly pol
ymorphic microsatellite or minisatellite loci. Loss of heterozygosity
of 1p was detected in 19 cases (33%) of primary tumors and in 21 cases
(36%) when metastatic and recurrent sites were included. They were cl
assified into two groups according to the 1p deletion pattern: interst
itial deletion (group I, n = 11) and terminal deletion (group T, n = 1
0). The shortest region of overlap in group I ranged between FGR and D
1S170 (1p36.1-2). Clinically, all group I cases survived disease free,
and none of these cases showed MYCN amplification. However, in group
T, eight (80%) cases showed a large terminal deletion front D1S162 (1p
32-pter), including the shortest region of overlap of group I, and two
(20%) showed a very terminal deletion from D1S160 (1p 36.3). Of the g
roup T cases, only two survived disease free, and seven (70%) showed M
YCN amplification. Thus, the candidates for the locations of neuroblas
toma suppressor genes on 1p may involve at least two regions, which de
monstrate different clinical features.