CD8(+) T cell knockout mice are less susceptible to Cowdria ruminantium infection than athymic, CD4(+) T cell knockout, and normal C57BL/6 mice

The role of T cells in immunity to Cowdria ruminantium was investigated by studying the responses to infection of normal, athymic, CD4(+) T cell knock out (KO) and CD8(+) T cell KO C57BL/6 mice. Normal C57BL/6 mice could be i mmunized by infection and treatment, and immunity was adoptively transferab le from immune to naive mice by splenocytes. Following infection, athymic m ice died sooner than normal mice (P = 0.0017), and could not be immunized b y infection and treatment. CD4(+) T cell KO mice were as susceptible to inf ection as normal mice and could be immunized by infection and treatment. In contrast, CD8(+) T cell KO mice were less susceptible than normal and CD4( +) T cell KO mice and 43% self-cured, while those that died did so after a prolonged incubation period. Antibody responses to C. ruminantium were CD4( +) T cell dependent, because responses were detected in immune normal and C D8(+) T cell KO mice but not in immune CD4(+) KO mice (P = 0.005). Since CD 8(+) T cell KO mice were less susceptible to infection, and since CD4(+) T cell KO mice could be immunized, it can be concluded that immunity to C. ru minantium can be mediated by both CD4(+) and CD8(+) T cells. (C) 2000 Elsev ier Science B.V. All rights reserved.