Formaldehyde toxicity during acetoclastic methanogenesis exerted a dual cha
racter. It was in part reversible and in part irreversible. Toxicity was on
ly reversible at very low formaldehyde concentration. The deg ee of irrever
sible toxicity was proportionally related to the initial sludge concentrati
on, but was independent of the initial specific acetoclastic activity. Form
aldehyde conversion seemed to be dependent on the concentration of the vari
ous trophic groups present in the anaerobic granular sludge used. Hence bio
mass diversity may play an important role in the outcome of toxicity studie
s and may furthermore contribute to the explanation of the controversies wi
th respect to toxicity values reported in literature.