dThe mineralization of the azo dye Disperse Blue 79 (DB79) was studied unde
r sequenced anaerobic/aerobic environments. This paper presents the results
of the first stage of the process, i.e., the anaerobic decolouration of th
e azo dye. A sequencing batch biofilter was used. The results showed that D
B79 was only biotransformed to amines, resulting in a decolorization of the
effluent. The anaerobic bacteria, without using co-substrate, achieved the
decolouration when the concentration of DB79 was lower than 48 mg/l. It wa
s necessary to use a co-substrate to reach decolouration efficiencies highe
r than 95%, when the concentration of DB79 in the influent was superior to
48 mg/l. In 72 h more than 95% of the initial colourant was biotransformed.