dTwo sequencing batch reactors (SBRs) with sequenced anaerobic/aerobic phas
es were used to study biological colour removal from a simulated cotton tex
tile effluent containing an azo reactive dye. One of the reactors was daily
fed with Remazol Brilliant Violet 5R dye and the other was used as control
. When operating with a sludge retention time (SRT) of 15 days the total CO
D removal was around 80%, with 30% being removed anaerobically. After 40-50
days of acclimatization the colour removal efficiency reached a maximum, s
table value of 90% from a feed dye concentration of 90 mg/l, almost all bei
ng removed during the anaerobic phase. This colour removal was attributed t
o microbial degradation rather than adsorption and colour removal capacity
was not lost even after a seven-day absence of dye in the fed substrate. Th
e dye-fed reactor experienced a reduction in the ORP values attained during
the non-aerated phase, after acclimatization, an effect not observed in th
e dye-free control. Under denitrifying conditions it was observed that the
decolouration levels achieved in the anaerobic phase decreased from 90% to
70% after only two cycles with a feed containing 45-60 mg NO3/l. Reduction
of the SRT value from 15 to 10 days reduced the biomass concentration from
2.0 to 1.2 g VSS/l and lowered colour removal levels from 90% to 30-50%. Wh
en the SRT value was increased back to 15 days the colour removal capacity
of the system was completely recovered, suggesting that with a SRT of 10 da
ys the adequate microbial population could not be installed in the reactors
.