This study consisted of two stages. The first stage was to cultivate denitr
ifying biofilm with a simplified UCBR while the second stage was to investi
gate the performance of a standard UCBR for nitrate removal. The volumetric
nitrate-N conversion rate increased steadily from less than 0.5 kg/m(3).d
to about 2.5 kg/m(3).d towards the end of Stage 1. Reactor instability and
poor biofilm morphology had led to a relatively low nitrate-N conversion ra
te during stage 1 of operation. In Stage 2, the conversion rate increased r
apidly to 6.5 kg/m(3).d within 4 days and increased steadily to 10.5 kg/m(3
).d, thereafter. The biofilm formation in Stage 2 was better than that atta
ined in Stage 1. Biofilm density and biomass concentration in the reactor w
ere in the range of 20.5 to 29.0 g/L and 1 to 3 g/L, respectively. The spec
ific growth rate of the denitrifiers (mu) and the observed biomass growth y
ield coefficient (Y-obs) in the reactor varied within the ranges of 1.5-4.2
d-1 and 0.20-0.65 g biomass/g nitrate-N, respectively. The specific nitrat
e-N conversion rate (gamma) was within the range of 1.8-3.8 g nitrate-N/g b
iomass.d.