Construction of fission yeast vectors with a novel selection strategy thatallows their use in wild-type fission yeasts

Novel vectors that use the Pichia pastor is INO1 gene as a selectable marke r and exploit the natural inositol auxotrophy of the fission yeast are desc ribed. These plasmids also contained other features desirable in a plasmid cloning vector. These plasmids were evaluated in other species of Schizosac charomyces and found to replicate autonomously in another variety of S. pom be, S. pombe var. malidevorans. These plasmids can be used for transformati on of any wild-type S. pombe strain without the need for selection by induc ed auxotrophic mutations, or by selection by drug resistance markers, and s hould be greatly assist genetic and molecular manipulations in these yeasts . Copyright (C) 2000 John Wiley & Sons, Ltd.