Atmospheric pressure MALDI/ion trap mass spectrometry

Citation
Vv. Laiko et al., Atmospheric pressure MALDI/ion trap mass spectrometry, ANALYT CHEM, 72(21), 2000, pp. 5239-5243
Citations number
10
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
00032700 → ACNP
Volume
72
Issue
21
Year of publication
2000
Pages
5239 - 5243
Database
ISI
SICI code
0003-2700(20001101)72:21<5239:APMTMS>2.0.ZU;2-2
Abstract
A new sample ionization technique, atmospheric pressure matrix-assisted las er desorption/ionization (AP MALDI), was coupled with a commercial ion trap mass spectrometer. This configuration enables the application-specific sel ection of external atmospheric ionization sources: the electrospray/APCI (c ommercially available) and AP MALDI (built in-house), which can be readily interchanged within minutes. The detection limit of the novel AP MALDI/ion trap is 10-50 fmol of analyte deposited on the target surface for a four-co mponent mixture of peptides with 800-1700 molecular weight. The possibility of peptide structural analysis by MS/MS and MS3 experiments for AP MALDI-g enerated ions was demonstrated for the first time.