Kinetic studies of imidazole and its methyl derivative binding to metmyoglobin: Effects of substitute methyl on the binding affinity

Authors
Qian, CM Yao, Y Wu, YB Tang, WX
Citation
Cm. Qian et al., Kinetic studies of imidazole and its methyl derivative binding to metmyoglobin: Effects of substitute methyl on the binding affinity, BIOMETALS, 13(3), 2000, pp. 267-272
Citations number
15
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOMETALS
ISSN journal
09660844 → ACNP
Volume
13
Issue
3
Year of publication
2000
Pages
267 - 272
Database
ISI
SICI code
0966-0844(200009)13:3<267:KSOIAI>2.0.ZU;2-C
Abstract
The binding of imidazole and imidazole derivatives (4- and 2-MeIm) to the m etMb was studied by H-1 NMR spectroscopy to elucidate the effects of differ ent methyl substitution positions on the affinity and kinetics of binding t o metMb. These exogenous ligands can form stable complexes with metMb excep t 2-MeIm. Kinetics and equilibrium data for the binding of imidazole and 4- MeIm to metMb have been obtained by 2D EXSY, and the reasons for the differ ent affinity are also discussed.