Distribution of ganglioside GM1 in L-alpha-dipalmitoylphosphatidylcholine/cholesterol monolayers: A model for lipid rafts

The distribution of low concentrations of ganglioside GM1 in L-alpha -dipal mitoylphosphatidylcholine (DPPC) and DPPC/cholesterol monolayers supported on mica has been studied using atomic force microscopy (AFM). The monolayer s studied correspond to a pure gel phase and a mixture of liquid-expanded ( LE) and liquid-condensed (LC) phases for DPPC and to a single homogeneous l iquid-ordered phase for 2:1 DPPC/cholesterol. The addition of 2.56% GM1 to phase-separated DPPC monolayers resulted in small round ganglioside-rich mi crodomains in the center and at the edges of the LC domains. Higher amounts of GM1 (10%) give numerous filaments in the center of the LC domains and l arger patches at the edges. A gel phase DPPC monolayer containing GM1 showe d large domains containing a network of GM1-rich filaments. The addition of GM1 to a liquid-ordered 2:1 DPPC/cholesterol monolayer gives small, round domains that vary in size from 50 to 150 nm for a range of surface pressure s. Larger amounts of GM1 lead to coalescence of the small, round domains to give longer filaments that cover 30-40% of the monolayer surface for 10 mo l % GM1. The results indicate that biologically relevant GM1 concentrations lead to submicron-sized domains in a cholesterol-rich liquid-ordered phase that is analogous to that found in detergent-insoluble membrane fractions, and are thought to be important in membrane microdomains or rafts. This de monstrates that AFM studies of model monolayers and bilayers provide a powe rful method for the direct detection of microdomains that are too small for study with most other techniques.