Sites of expression of DnaJ homologs and Hsp70 in male and female flowers of the Japanese willow Salix gilgiana seemen

We did in situ hybridization to identify the sites at which DnaJ homologs ( SGJ1 and SGJ3) and Hsp70 were expressed in male and female flowers of the J apanese willow, Salix gilgiana Seemen. Transcripts of SGJ1, SGJ3, and Hsp70 were detected in microspores and in the tapetal layers of immature anthers , in the inner integument of ovules, and in the funicular epidermal layers of ovaries. Transcripts of SGJ3 and Hsp70 were also detected in the nectari es of male and female flowers, in which transcripts of SGJ1 were undetectab le.