K. Hirota et al., Interaction of local anaesthetics with recombinant mu, kappa, and delta-opioid receptors expressed in Chinese hamster ovary cells, BR J ANAEST, 85(5), 2000, pp. 740-746
Citations number
38
Categorie Soggetti
Aneshtesia & Intensive Care","Medical Research Diagnosis & Treatment
Local anaesthetics potentiate epidural or intrathecal opioid analgesia via
a poorly defined mechanism. In this study, we have examined the interaction
of local anaesthetics (lidocaine, bupivacaine and its optical isomers, tet
racaine, procaine and prilocaine) with recombinant mu-, kappa-, and F-opioi
d receptors expressed in Chinese hamster ovary cells (CHO-mu, kappa, and de
lta, respectively). Lidocaine produced a concentration-dependent displaceme
nt of radiolabelled opioid antagonist [H-3]diprenorphine ([H-3]DPN) binding
with the following rank order of inhibitor constant (Ki): kappa (210 muM)
> mu (552 muM) > delta (1810 muM). Procaine, prilocaine, tetracaine and bup
ivacaine also displaced [H-3]DPN binding in CHO- mu with K-i values of 244,
204, 43 and 161 muM respectively. Lidocaine produced a concentration-depen
dent and naloxone-insensitive inhibition of cAMP formation in all cell line
s including untransfected cells. Concentration producing 50% inhibition of
maximum was mu, 1.32 mM; kappa-, 2.41 mM; delta, 1.27 mM; untransfected, 2.
78 mM. When lidocaine (300 muM) was co-incubated with spiradoline (kappa -s
elective) and [D-Ala(2), MePhe(4), Gly(ol)(5)] enkephalin (DAMGO mu -select
ive) in CHO-kappa and mu cells we did not observe an additive interaction f
or cAMP formation. In contrast, there was an apparent inhibitory action of
the combination at the kappa receptor. This study suggests that clinical co
ncentrations of local anaesthetics interact with mu and kappa but not delta
opioid receptors. As there was no synergism between local anaesthetics and
opioids we suggest that the interaction of these agents in the clinical se
tting does not occur at the cellular level.