A survey of 16S rRNA and amoA genes related to autotrophic ammonia-oxidizing bacteria of the beta-subdivision of the class proteobacteria in contaminated groundwater

In this study, we investigated the size and structure of autotrophic ammoni a oxidizer (AAO) communities in the groundwater of a contamination plume or iginating from a mill-tailings disposal site. The site has high levels of d issolved N from anthropogenic sources, and exhibited wide variations in the concentrations of NO3- and NH3 + NH4+. Community structures were examined by PCR-DGGE targeting 16S rDNA with band excision and sequence analysis, an d by analysis of amoA fragment clone libraries. AAO population sizes were e stimated by competitive PCR targeting the gene amoA, and correlated signifi cantly with nitrate concentration. Most samples revealed novel diversity in AAO 16S rDNA and amoA gene sequences. Both 16S rDNA and amoA analyses sugg ested that all samples were dominated by Nitrosomonas sp., Nitrosospira sp. being detected in only 3 of 15 samples. This study indicated numerical dom inance of Nitrosomonas over Nitrosospira in groundwater, and suggests that groundwater ammonia oxidizers are more similar to those dominating freshwat er sediments than bulk soil.