F. Dodero et al., Quantitative analysis of glycogen content in hepatocytes of human liver allograft after ischemia and reperfusion, CELL MOL B, 46(7), 2000, pp. 1157-1161
In order to examine glucose metabolism in liver grafts after cold ischemia
and reperfusion, the heterogeneous lobular distribution pattern of glycogen
content was studied using histochemical quantitative analysis. In most of
the cases, this heterogeneous pattern of glycogen was observed after preser
vation and reperfusion. However, a 42% reduction of glycogen content, expre
ssed as the ratio between stained surface and total surface of liver biopsi
es, was observed in biopsies after reperfusion. Moreover, both periportal a
nd centrilobular hepatocytes showed a significant decrease in mean optical
density after reperfusion (18% and 25%, respectively). The comparison of ou
r results to early postoperative liver function tests and cold ischemia tim
es showed no significant correlation (p<0.05).