Invertase production of common storage moulds in food and feed grains as apossibility for rapid detection of aspergillus flavus group and Aspergillus fumigatus
T. Matrai et al., Invertase production of common storage moulds in food and feed grains as apossibility for rapid detection of aspergillus flavus group and Aspergillus fumigatus, INT J F MIC, 61(2-3), 2000, pp. 187-191
Invertase production of grain storage moulds was studied. Aspergillus spp.
and Penicillin spp. were grown in a sucrose based liquid medium, at 37 degr
eesC. The A. flavus group (A. flavus, A. parasiticus, A. nomius, A. oryzae)
and A. fumigatus showed a fast growth and intense invertase activity, whil
e other Aspergillus spp. and Penicillium spp. grew slower and produced less
invertase. The pattern of accumulated reducing sugar after 20 and 48 h of
incubation was characteristic to the species studied. From inoculation stud
ies the detection limit was calculated as: 1-10 conidia of A. flavus group
and A. fumigatus, as compared to 10(3)-10(4) for the other species studied.
The method may be recommended as a rapid test for the detection of A. flav
us group and A. fumigatus in food and feed grains. (C) 2000 Elsevier Scienc
e B.V. All rights reserved.