We have conducted a series of experiments in order to test hypothesis about
the possible use of nitrogen fixation by small phytophagous mammals as a w
ay to supplement their nitrogen balance. Two groups of red voles Clethriono
mys rutilis were kept on rations with different protein nitrogen content. T
hree days later, the rate of nitrogen fixation was determined in different
regions of the digestive tract. Nitrogen-fixing activity has been detected
in the GI tract of all studied voles, specifically in those regions, where
symbiocenoses were present; it was particularly high in animals of the expe
rimental group under the conditions of dietary nitrogen limitation. A mixed
culture of bacteria capable of nitrogen fixation has been isolated from th
e digestive tract of red voles.