Clarithromycin resistance stability in Helicobacter pylori: influence of the MIC and type of mutation in the 23S rRNA

Citation
T. Alarcon et al., Clarithromycin resistance stability in Helicobacter pylori: influence of the MIC and type of mutation in the 23S rRNA, J ANTIMICRO, 46(4), 2000, pp. 613-616
Citations number
10
Categorie Soggetti
Pharmacology,Microbiology
Journal title
Journal of antimicrobial chemotherapy
ISSN journal
03057453 → ACNP
Volume
46
Issue
4
Year of publication
2000
Pages
613 - 616
Database
ISI
SICI code
Abstract
Thirty clarithromycin-resistant Helicobacter pylori strains (MIC range 8-64 mg/L) were subcultured in a drug-free medium and the MIC was determined ev ery five passages to detect in vitro stability of resistance. Three out of the 30 (10%) lost their resistance after 10, 13 or 18 subcultures (MIC decr ease from 8 to 0.008, from 16 to 0.064 and from 32 to 0.016 mg/L). The effe ct of four macrolides at subinhibitory concentrations on the development of resistance was studied in H. pylori NCTC 11638 and TIGR 26695. A change in the MIC was observed only when NCTC11638 was exposed to 0.5 x MIC of eryth romycin for 20 days.